Journal: British Journal of Nutrition

Article Title: Identification of epicatechin as one of the key bioactive constituents of polyphenol-enriched extracts that demonstrate an anti-allergic effect in a murine model of food allergy

doi: 10.1017/s0007114514000877

Figure Lengend Snippet: Fig. 1. Ability of two different polyphenol-enriched apple extracts to affect allergy symptoms in sensitised mice. (a) Ovalbumin (OVA)-induced murine model of food allergy and timing of dietary intervention. (b) Allergy symptom scores after intervention with polyphenol-enriched apple extracts. (c) Distribution of allergy symptom scores (scale 0–4) in the different groups (0, ; 1, ; 2, ; 3, ; 4, ), with 4 being the score indicating the severest symptom. (d) Drop in temperature (8C) recorded in the different groups before and after the challenge. (e) Concentration of OVA-specific IgG2a (mg/ml) in the different groups. Groups are rep- resented as negative control (Neg cont, ), positive control (Pos cont, ), apple extract A ( ) and apple extract B ( ). ** Mean value was significantly different from that of the positive control group (P,0·01).

Article Snippet: Serially diluted standard (monoclonal mouse anti-OVA IgG1 and monoclonal mouse anti-OVA IgG2a from Antibody Shop; Lucerna-Chem) and sera were incubated for 2 h at 378C, followed by incubation for 2 h with a HRP labelled goat antimouse IgG1 or IgG2a antibody (1:5000; Southern Biotech; Bioconcept).

Techniques: Concentration Assay, Negative Control, Positive Control

Journal: Frontiers in cell and developmental biology

Article Title: RNA-binding is an ancient trait of the Annexin family.

doi: 10.3389/fcell.2023.1161588

Figure Lengend Snippet: FIGURE 1 AnxA1, AnxA2, AnxA4, AnxA5, AnxA6, AnxA7, AnxA10, AnxA11 and AnxA13 present in the cytoskeleton fraction (Panel A) are associated with non-polysomal mRNP complexes (Panel B) of PC12 cells. Panel (A) 30 µg of the cytoskeletal fraction (lane 1) and cytoskeleton-bound polysomes (lane 2) were separated by 10% SDS-PAGE and transferred to nitrocellulose membranes. Panel (B) samples prepared from oligo (dT)-bound mRNP complexes from the cytoskeletal fraction [supernatant after centrifugation for 2 h 100,000 g above a 1 M (35%) sucrose cushion] (lanes 3 and 4), without (lane 3) or with RNase (lane 4) treatment, as indicated above the Western blots, were subjected to similar analysis. Theblots were probed with antibodies against the different Anxs and against PABP1 as a marker for poly(A)-containing mRNAs, as indicated. Antibodies against the ribosomal subunit S6 were used to inform of the distribution of ribosomes. In addition, the blots were probed with antibodies against early endosomes (EEA1), late endosomes (Rab7) and recycling endosomes (Rab11). SPC25 and LAMP1 were not detectable in any of the fractions (results not shown). Visualization of the immunoreactive protein bands was performed using the ChemiDocTM XRS+ molecular imager after incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies and enhanced chemiluminescence (ECL)-reagent. The blots shown are representative for results from three experiments.

Article Snippet: Proteins were detected by incubation at a 1:2,000 dilution with secondary horseradish peroxidase (HRP)-conjugated antibodies (170–6,516 (goat anti-mouse) or 170–6,515 (goat anti-rabbit) from Bio-Rad, Hercules, United States) Frontiers in Cell and Developmental Biology frontiersin.org06 or from Santa Cruz, Dallas, United States (sc-2020; donkey anti-goat).

Techniques: SDS Page, Centrifugation, Western Blot, Marker, Incubation